Dynamic Islet Culture Platform for High-throughput Drug Screening
نویسندگان
چکیده
Currently, we are heading towards the period of nanotechnology which extends to many engineering disciplines. The necessity of its development is crucial to make life more convenient, to improve efficiency of each research field, and to integrate portable healthcare system. Thus, the next most important step is to develop a technology to meet the needs of both industry and research area [1]. Here, we developed a fully integrated microfluidic device to perform the dynamic islet culture used for multiple drug toxicity tests while simultaneously characterizing their functionality through fluorescence imaging of the mitochondrial membrane potential and intercellular calcium influx with enzyme linked immunosorbent assay for quantification of secreted insulin concentration. The dynamic islet culture platform made of triple layer is composed of a comb-patterned micromixer, a media distributor, four perfusion chambers, cell arrays and microfluidic channels for cell and drug injection and collection.
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